Yeast protocols handbook clontech

See more. Amberg , , Springer Protocols. Next-generation sequencing to generate interactome datasets Haiyuan Yu et al. References Fields, S. Nature , — Chien, C. USA 88 , — Sambrook, J. Birnboim, H.

Nucleic Acids Res. Y yeast transformation system 2 manual The yeast two-hybrid system is a powerful tool for screening protein-protein interactions and has also been used for large-scale studies. We evaluated two protein-coding sequences as reporter genes for the yeast two-hybrid system, to determine if it was suitable as an alternative screening strategy. The Matchmaker Gold Yeast Two-Hybrid System is the most advanced and highest performing version of our highly popular Matchmaker product line for investigating protein-protein interactions.

This is due to a combination of a new yeast strain Y2H Gold , stringent reporters, easy-to-use libraries, high-level expression vectors, and SMART-based library construction kits. Y yeast competent cells were prepared using the Yeastmaker Yeast Transformation System 2 kit according to the user manual.

To calculate the number of Yeast Media Set 2 Cat. Yeast Protocols Handbook Protocol No. PT www. Version No. PR 5 II. Introduction to Yeast Promoters Yeast promoters and other cis-acting regulatory elements play a crucial role in yeast-based expression systems and transcriptional assays such as the Matchmaker One- and Two-Hybrid Systems B.

During the latter half of the s, first spheroplasts transformation method was established in the yeast Saccharomyces cerevisiae. With the advent of synthetic biology and genome technologies, which require handling of very large sized DNA molecules, the spheroplast method has once again become important. More than 4, individual colonies containing potential interactors were identified.

The primary benefits of utilizing this mating protocol The Yeast Two Hybrid system: A tool for mapping protein-protein interactions. Genes Dev — Sheerin DJ Investigation of light-regulated protein-protein interactions using yeast two-hybrid assays. In: Hiltbrunner A ed Phytochromes: methods and protocols. Springer New York, New York, pp 1— Nat Biotechnol — J Biol Eng Nucleic Acids Res — Mol Biol Cell — Download references. You can also search for this author in PubMed Google Scholar.

HL and JL designed research. JL and HL analyzed the data. JL and HL wrote the manuscript. Correspondence to Jigang Li. Reprints and Permissions. Li, H. A LexA-based yeast two-hybrid system for studying light-switchable interactions of phytochromes with their interacting partners.

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